HetaSep™

HetaSep™

For depletion of red blood cells from fresh blood samples and isolation of nucleated cells

脐带血是临床移植中造血干/祖细胞（HSPCs）的主要来源。每个脐血单位中HSPCs的数量不多，并且单位之间所含细胞数量的差异也很大。细胞的处理和冻存也会影响活干/祖细胞的数量和质量1。因此，使用测定脐血单位“效价”的检测方法来确定该单位的HSPC含量至关重要。我们推荐从样本中去除红细胞（RBCs），因为红细胞的存在会干扰效价的检测方法导致对样本不正确的评估。从小样本（如附接在主脐血单位上的小段）中去除红细胞的方法包括：免疫磁珠去除、沉降去除或氯化铵裂解。

概要

HetaSep™ is an erythrocyte aggregation agent used to quickly separate nucleated cells from red blood cells (RBCs) in whole blood. Aggregated erythrocytes settle much faster than dispersed cells. By controlling the settling time and/or centrifugation speed, the majority of nucleated cells are recovered in the supernatant. Approximately 95 - 99% RBC depletion is attained if the nucleated cell-rich fraction is removed carefully.

Components:

Contains:

• Hetastarch (6% w/v)

• Sodium chloride

• Sodium lactate (anhydrous)

• Dextrose (hydrous)

• Calcium chloride dehydrate

• Potassium chloride

• Magnesium chloride hexahydrate

• Other ingredients

Subtype:

细胞分选试剂盒

Cell Type:

粒细胞及其亚型; 红细胞

Species:

人

Sample Source:

脐带血; 全血

Selection Method:

负选

Application:

细胞分离

Brand:

HetaSep

Area of Interest:

免疫学; 干细胞生物学

生产厂家：加拿大stemcell

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