DMEM / Ham’s F-12

德国SERANA系列DMEM / Ham’s F-12

Serana

Serana Europe GmbH is a leading manufacturer and supplier of cell culture products. Our product range includes animal & human sera, classical & serum free media as well as liquid & powdered media, reagents,supplements, buffer solutions for cell culture and diagnostic applications. Serana’s products are used in all areas where cell culture is performed. This includes the biopharmaceutical industry for the production of vaccines, therapeutic proteins and diagnostics. In addition, we are a major supplier to the Academic R&D (universities, hospitals & clinics), private research institutes and small biotech companies.

DMEM / Ham’s F-12

Specifications

Liquid Product Specifications	Powder Product Specifications
Appearance Sterility pH Osmolality Endotoxin Mycoplasma Cell Culture Storage	Clear, Red Solution Tested 7.00 – 7.50 260 – 320 mM/Kg ≤ 1.0 EU/mL Tested Tested +2°C to +8°C	Appearance Solubililty Moisture Content Particle Size (100 mesh) pH without NaHCO3 pH with 3700 mg/L NaHCO3 Osmolality without NaHCO3 Osmolality with 3700 mg/L NaHCO3 Endotoxin	Red to Orange Powder Pass ≤ 2 % ≥ 90 % Record 6.5 – 8.0 Record mM/Kg 260 - 320 mM/Kg ≤ 1.0 EU/mL

Note: NaHCO3 is not included in the powder media formulation.

Formulation

Components	mg/L	Components	mg/L
Biotin (C10H16N2O3S)	0.0035	L-Serine (C3H7NO3)	26.2500
Calcium Chloride, Anhydrous (CaCl2)	116.6000	L-Threonine (C4H9NO3)	53.4500
Choline Chloride	8.9800	L-Tryptophan (C11H12N2O2)	9.0200
Cupric Sulfate, Pentahydrate (CuSO4 . 5H2O)	0.0013	L-Tyrosine, Disodium, Dihydrate (C9H9NO3Na2 . 2H2O)	55.7900
D-Calcium Pantothenate (C18H32CaN2O10)	2.2400	L-Valine (C5H11NO2)	52.8500
D-Glucose, Anhydrous (C6H12O6)	3151.0000	Linoleic Acid (C18H32O2)	0.0420
Ferric Nitrate, Nonahydrate (Fe(NO3)3 . 9H2O)	0.0500	Lipoic Acid (DL-Thiotic Acid) (C8H14O2S2)	0.1050
Ferrous Sulfate, Heptahydrate (FeSO4 . 7H2O)	0.4170	Magnesium Chloride, Anhydrous (MgCl2)	28.6100
Folic Acid (C19H19N7O6)	2.6500	Magnesium Sulfate, Anhydrous (MgSO4)	48.9300
Glycine (C2H5NO2)	18.7500	Myo-Inositol (C6H12O6)	12.6000
Hypoxanthine, Sodium (C5H3N4O.Na)	2.3900	Niacinamide (C6H6N2O)	2.0200
L-Alanine (C3H7NO2)	4.4500	Phenol Red, Sodium Salt (C19H13NaO5S)	8.1000
L-Arginine, Hydrochloride (C6H14N4O2 . HCl)	147.5000	Potassium Chloride (KCl)	311.8000
L-Asparagine, Monohydrate (C4H8N2O3 . H2O)	7.5000	Putrescine, Dihydrochloride (C4H12N2 . 2HCl)	0.0810
L-Aspartic Acid (C4H7NO4)	6.6500	Pyridoxine, Hydrochloride (C8H11NO3 . HCl)	2.0310
L-Cysteine, Hydrochloride, Monohydrate (C3H7NO2 . HCl . H2O)	17.5600	Pyruvic Acid, Sodium Salt (C3H3NaO3)	55.0000
L-Cystine, Dihydrochloride (C6H12N2O4S2 . 2HCl)	31.2900	Riboflavin (C17H20N4O6)	0.2190
L-Glutamic Acid (C5H9NO4)	7.3500	Sodium Bicarbonate (NaHCO3)	2438.0000
L-Glutamine (C5H10N2O3)	365.0000	Sodium Chloride (NaCl)	6995.5000
L-Histidine, Hydrochloride, Monohydrate (C6H9N3O2 . HCl . H2O)	31.4800	Sodium Phosphate Dibasic, Anhydrous (Na2HPO4)	70.9600
L-Isoleucine (C6H13NO2)	54.4700	Sodium Phosphate, Monobasic, Monohydrate (NaH2 PO4 . H2O)	62.5000
L-Leucine (C6H13NO2)	59.0500	Thiamine, Hydrochloride (C12H17ClN4OS . HCl)	2.1700
L-Lysine, Hydrochloride (C6H14N2O2 . HCl)	91.2500	Thymidine (C10H14N2O5)	0.3650
L-Methionine (C5H11NO2S)	17.2400	Vitamin B12 (C63H88CoN14O14P)	0.6800
L-Phenylalanine (C9H11NO2)	35.4800	Zinc Sulfate, Heptahydrate (ZnSO4 . 7H2O)	0.4320
L-Proline (C5H9NO2)	17.2500

Ordering Information

Liquid Product Code	Powder Product Code	Description	Liquid Volume	Powder Makes
MCL-021-500ML	MCP-021-50L	With stabilized L-glutamine.	500ml	50L
MCL-022-500ML	MCP-022-50L	With L-glutamine and 15mM HEPES. Without choline chloride and phenol red.	500ml	50L
MCL-023-500ML	MCP-023-50L	With L-glutamine and 15 mM HEPES.	500ml	50L
MCL-024-500ML	MCP-024-50L	With L-glutamine and 15mM HEPES. Without phenol red.	500ml	50L
MCL-025-500ML	MCP-025-50L	With L-glutamine and sodium pyruvate. Without HEPES.	500ml	50L
MCL-026-500ML	MCP-026-50L	With sodium pyruvate. Without L-glutamine and HEPES.	500ml	50L
MCL-027-500ML	MCP-027-50L	With L-glutamine. Without L-arginine and L-lysine.	500ml	50L
MCL-028-500ML	MCP-028-50L	With stabilized L-glutamine. Without L-arginine, L-lysine, and L-methionine	500ml	50L

生产厂家：德国Serana Europe GmbH

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