Roche  11277073910  DIG RNA Labeling Mix

公司简介

罗氏总部位于瑞士巴塞尔，在制药和诊断领域是世界领先的以研发为基础，以创新为驱动的健康医疗公司之一。作为全球**的生物技术公司之一，罗氏在抗肿瘤、抗病毒、炎症、代谢和中枢神经系统领域拥有切实有效的差异化药品；同时罗氏也是体外诊断和基于组织的肿瘤诊断的市场***，以及糖尿病管理领域的先驱者。罗氏倡导的“个性化医疗”方案旨在提供创新的药品和诊断工具，从而切实改善人类的健康水准、生活质量以及患者的生存期。

在成功收购德国宝灵曼公司（Boehringer Mannheim GmbH.）后，成立了专业服务于科学研究领域用户的罗氏诊断生命科学部（Roche Life Science），凭借半个多世纪在生命科学研究领域积累的经验，罗氏诊断生命科学部已成为在此领域中具重要地位的试剂及系统供应商之一。罗氏诊断生命科学部还为生命科学行业、生物制药行业和诊断行业提供所需的原料、试剂组分和试剂盒，罗氏诊断生命科学部为客户进行量身定制产品，满足客户个性化需求。

产品介绍

Features and Benefits

• The DIG RNA Labeling Mix is especially designed for the use with SP6,T7 and T3 RNA polymerases from Roche which are supplied with an optimized transcription buffer.

General description

• Labeling efficiency: Approximately 10μg of full-length digoxigenin-labeled RNA is transcribed from 1μg linear template DNA.Assay Time: 135 minutesSample MaterialsLinearized plasmid DNA:The DNA to be transcribed is cloned into the polylinker site of an appropriate transcription vector which contains adjacent to the polylinker a promoter for SP6, T7 or T3 RNA polymerase. For the synthesis of ‘run off’ transcripts the plasmid is linearized by a restriction enzyme. Restriction enzymes creating 5′-overhangs should be used; 3′ overhangs should be avoided. The linearized template DNA should be purified by phenol chloroform extraction and ethanol precipitation, to avoid RNase contamination. For ′run around′ transcription circular plasmid DNA is used.PCR product:PCR-fragments which contain RNA polymerase promoter sequences can also be used as templates for transcription. Purification of the PCR fragment by HighPure column purification prior to transcription is recommended.
• DIG-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. DIG-11-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The DIG RNA Labeling Mix is specifically designed for the use with SP6, T7, and T3 RNA polymerases, which are supplied with an optimized transcription buffer.Convenient nucleotide mixture for the labeling of RNA with Digoxigenin-11-UTP.Contents10x solution with: 10 mM ATP, CTP, GTP (each), 6.5 mM UTP, 3.5 mM DIG-11-UTP.
• Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Other Notes

• 仅用于生命科学研究。不可用于诊断。

Quality

• Function tested in the DIG RNA Labeling Kit and in the DIG Nucleic Acid Detection Kit.

Specificity

• Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0).

Application

• RNA labeling with Digoxigenin-11-UTP by in vitro transcription with SP6, T7, and T3 RNA polymerases. DIG-labeled RNA is used in a variety of hybridization techniques: • Northern blots^[4]• Southern blots• Dot blots• Plaque or colony lifts• RNase protection experiments• Chromosomes, cells, and tissue sections in situ^[6][3]

性质




Related Categories	12 Principles Aligned Products, Chemical Synthesis,Greener Alternative Products, Life Sciences
form	solution
usage	sufficient for 20 reactions
packaging	pkg of 40 μL
mfr. no.	Roche
greener alternative product characteristics	Designing Safer ChemicalsLearn more about the Principles of Green Chemistry.
shipped in	dry ice
storage temp.	−20°C




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