在成功收购德国宝灵曼公司（Boehringer Mannheim GmbH.）后，成立了专业服务于科学研究领域用户的罗氏诊断生命科学部（Roche Life Science），凭借半个多世纪在生命科学研究领域积累的经验，罗氏诊断生命科学部已成为在此领域中具重要地位的试剂及系统供应商之一。罗氏诊断生命科学部还为生命科学行业、生物制药行业和诊断行业提供所需的原料、试剂组分和试剂盒，罗氏诊断生命科学部为客户进行量身定制产品，满足客户个性化需求。
产品介绍
Features and Benefits
• Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer.• Complete protection: Instantly protect your proteins against a broad range of proteases (see Table 1).• Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.• Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
General description
cOmplete, Mini, EDTA-free tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other cOmplete tablets they do not contain EDTA, leaving the stability and the function of metal-dependant proteins unaffected. The affinity purification of Poly-His-tagged fusion proteins using IMAC (Immobilized Metal Affinity Chromatography) is also facilitated (no dialysis necessary).Due to the optimized composition of the tablets they show excellent inhibition of serine and cysteine proteases, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria. cOmplete, Mini, EDTA-free tablets, contains both irreversible and reversible protease inhibitors. Metallo- and aspartic proteases are not inhibited.cOmplete Mini, EDTA-free Tablets are identical to cOmplete Mini Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.
Other Notes
仅用于生命科学研究。不可用于诊断。
包装
30 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters. Each tablet is sufficient for a volume of 10 ml solution.
Preparation Note
Working concentration:1 tablet per 10 ml extraction solutionWorking solution:HandlingCarefully push the tablet through the foil packaging using the base of your thumb (not fingernail) to prevent the breakage of tablets.Preparation of Working SolutionOne cOmplete Mini EDTA-free tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively a stock solution (7 X conc.) can be prepared.Stock solution (7 X conc.)Dissolve one cOmplete Mini EDTA-free tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.Storage conditions (working solution):The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.
Quality
Performance-tested with pancreas extract.
Reconstitution
Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C, or at least 12 weeks at -15 to -25 °C. Can be used in thiol-containing solutions at 15 to 25 °C.
Specificity
Mixture of several protease inhibitors. For the inhibition of serine, cysteine, but not metalloproteases.
Physical form
Tablets in foil blister packs
Application
cOmplete™, Mini, EDTA-free, is used for the inhibition of serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts.[3][1][2]cOmplete, Mini, EDTA-free Tablets, are used for the inhibition of proteolytic activity in small volumes (up to 10 ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, Mini, EDTA-free, is preferentially used in the isolation process of Poly-His-tagged fusion proteins.If it is necessary to inhibit proteolytic activity in a large volume (up to 50 ml), we recommend to use cOmplete, EDTA-free.
