在成功收购德国宝灵曼公司（Boehringer Mannheim GmbH.）后，成立了专业服务于科学研究领域用户的罗氏诊断生命科学部（Roche Life Science），凭借半个多世纪在生命科学研究领域积累的经验，罗氏诊断生命科学部已成为在此领域中具重要地位的试剂及系统供应商之一。罗氏诊断生命科学部还为生命科学行业、生物制药行业和诊断行业提供所需的原料、试剂组分和试剂盒，罗氏诊断生命科学部为客户进行量身定制产品，满足客户个性化需求。
产品介绍
Features and Benefits
• Protect your precious proteins.Choose cOmplete ULTRA for even more efficient protease inhibition in eukaryotic, yeast, and bacterial cell lysates.• Why settle for less.Benefit from 7 powerful protease inhibitors – more than in other EDTA-free inhibitor tablets.• Defend against a broad range of proteases.Reliably inhibit serine, cysteine, and aspartic proteases.• Convenient and safe tablet format.Eliminate weighing small amounts of individual inhibitors which might be hazardous. Simply drop a tablet from the blister into your buffers.
General description
Recombinant proteins may be partially degraded during expression. Soluble degradation products are co-purified and cause a non-homogeneous protein preparation with protein fragments of different lengths. However, metalloproteases are prevalent in (for example) bacteria and some techniques require that EDTA be omitted.cOmplete ULTRA Tablets, Mini, EDTA-free contain the most powerful protease inhibitor cocktail for the optimal protection of your proteins against protease degradation. They are supplied as tablets in an EASYpack blister pack. EASYpacks provide the most convenient form of delivery - single tablets are easily pushed through the blister foil directly into the buffer. One cOmplete ULTRA Tablet, Mini, EDTA-free is sufficient for a volume of 10 ml extraction solution.cOmplete ULTRA Tablets, Mini, EDTA-free are identical to cOmplete ULTRA Tablets, Mini, the only difference being that no EDTA or other chelating agents (e.g.EGTA) are included.
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
Working concentration:1 tablet per 10 ml extraction solutionWorking solution:HandlingCarefully push the tablet through the foil packaging using the base of your thumb (not your fingernail) to prevent the breakage of tablets.Preparation of Working SolutionOne tablet is sufficient for the inhibition of proteolytic activity in 10 ml extraction solution. If very high proteolytic activity is present, 2-3 tablets should be used for 10 ml extraction buffer. The tablets can be added directly to the extraction medium.Stock Solution (2x conc.)Alternatively to the direct use with lysates, a 2x stock solution can be prepared, with the following procedure:• Add one cOmplete ULTRA Tablet, Mini, EDTA-free to 5 ml double-distilled water in a PE or PP tube.• Vortex 3 min or until the tablet is completely dissolved.• The stock solution is ready to use even if there are very fine particles still visible in solution. These particles do not interfere with the inhibition performance of the tablets.• This stock solution is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.• During storage of the 2x stock solution, the particles can sediment. Briefly vortex the stock solution prior to use.Storage conditions (working solution):The stock solution in water is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
Reconstitution
Soluble in aqueous buffers, or add directly to extraction media. For the stock solution, use water only.
Physical form
Tablets in foil blister packs. Each tablet is sufficient for a volume of 10 ml extraction solution.
Application
During cell lysis and purification, target proteins are at risk of being cleaved by proteases, which are naturally present in cells. To ensure that full-length proteins are obtained, cOmplete™ULTRA Tablets are used for the inhibition of an extended range of proteases. This new formulation shows highest inhibition ratios due to its broadened range of inhibitors compared to standard cOmplete Tablets.This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine and cysteine proteases. cOmplete ULTRA Tablets were developed for maximum efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.[2][1][3]This tablet does not contain EDTA; therefore, metalloproteases are not inhibited.For the purification of histidine-tagged proteins using Immobilized Metal Ion Affinity Chromatography (IMAC), this EDTA-free tablet is recommended for applications using non-EDTA compatible IMAC resins. Alternatively,an EDTA-containing cOmplete ULTRA, Mini tablet may be used in combination with Roche ′s new EDTA-compatible chromatography material, cOmplete His-Tag Purification Resin.
