BD Pharmingen™ 561969 FITC Mouse Anti-Human CD90
产品编号 561969 |BD Pharmingen™
Clone 5E10 (RUO)
产品描述
Brand
BD Pharmingen™
Alternative Name
THY1; Thy-1 antigen; Thy-1 membrane glycoprotein
Concentration
0.5 mg/ml
Isotype
Mouse BALB/c IgG1, κ
Reactivity
Human (QC Testing) Rhesus, Cynomolgus, Baboon, Pig, Dog (Tested in Development)
Application
Flow cytometry (Routinely Tested)
Immunogen
Human HEL Cell Line
Workshop No.
V M07, BP222; VI BP28, E046
Storage Buffer
Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status
RUO
产品详情
DESCRIPTION
- The 5E10 monoclonal antibody specifically binds to human CD90 which is also known as Thy-1. CD90 is a 25-35 kDa glycophosphatidylinositol-anchored membrane glycoprotein of the Ig superfamily that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. The anti-CD90 antibody binds to a subset of immature CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is highly enriched for cells capable of long-term culture. The anti-CD90 antibody is useful for enriching high proliferative potential colony-forming cells (HIPP-CFC) that are primative progenitor cells.
- This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
FORMAT
Format
FITC
Excitation Source
Blue 488 nm
Excitation Max
494 nm
Emission Max
520 nm
- FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
Other Formats →
APC
BUV395
BV421
BV510
BV605
Biotin
NA/LE
PE
PE-CF594
PE-Cy™5
PE-Cy™7
PerCP-Cy™5.5
Purified
SUGGESTED COMPANION PRODUCTS
Cat No. | Description | Size |
| | 100 Tests |
RESOURCES & TOOLS
Spectrum Viewer
Panel Designer
Download TDS
PREPARATION AND STORAGE
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
PRODUCT NOTICES
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
公司简介
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BD公司的业务共分为三大类: BD医疗, BD生物科学, BD诊断
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