BD Pharmingen™ 561014 APC Mouse Anti-Human CD105
产品编号 561014 |BD Pharmingen™
Clone AD2 (RUO)
产品描述
Brand
BD Pharmingen™
Alternative Name
NT5E; 5' nucleotidase; 5'-NT; E5NT; Ecto-5'-nucleotidase; eN; eNT; NT; NT5
Vol. Per Test
20 µl
Isotype
Mouse IgG1, κ
Reactivity
Human (QC Testing)
Application
Flow cytometry (Routinely Tested)
Immunogen
Pre-B leukemia cell line
Workshop No.
V B-CD73.3
Storage Buffer
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status
RUO
产品详情
DESCRIPTION
- The AD2 monoclonal antibody specifically binds to ecto-5’-nucleotidase, a 70 kDa, glycosyl phosphatidylinositol (GPI)-anchored glycoprotein. CD73 is expressed on subsets of T and B lymphocytes, follicular dendritic cells, epithelial cells, endothelial cells and mesenchymal stem cells. Its expression on lymphocytes increases during T and B cell development. CD73 has enzymatic activity and catalyzes the dephosphorylation of adenosine monophosphate (AMP) converting it to adenosine. It has been suggested that CD73 can mediate costimulatory signals in T cell activation and adhesion of lymphocytes to endothelium.
FORMAT
Format
PE
Excitation Source
Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
Excitation Max
496 nm
Emission Max
578 nm
- R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Other Formats
APC
BB515
BUV737
BV421
BV510
BV605
FITC
PE-CF594
PE-Cy™7
PerCP-Cy™5.5
Purified
V450
SUGGESTED COMPANION PRODUCTS
Cat No. | Description | Size | |
| | 100 Tests | |
RESOURCES & TOOLS
Spectrum Viewer
Panel Designer
Download TDS
PREPARATION AND STORAGE
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
PRODUCT NOTICES
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
公司简介
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BD公司的业务共分为三大类: BD医疗, BD生物科学, BD诊断
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