BD Pharmingen™ 560976 FITC Mouse Anti-Human CD45
产品编号 560976 |BD Pharmingen™
Clone HI30 (RUO)
产品描述
Brand
BD Pharmingen™
Alternative Name
PTPRC; CD45R; LCA; L-CA; Leukocyte Common Antigen; B220; T200; GP180; LY5
Vol. Per Test
20 µl
Isotype
Mouse IgG1, κ
Reactivity
Human (QC Testing)
Application
Flow cytometry (Routinely Tested)
Immunogen
Human Peripheral Blood Leucocytes
Workshop No.
IV N816
Storage Buffer
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status
RUO
产品详情
DESCRIPTION
- The HI30 monoclonal antibody specifically binds to the 180, 190, 205, 220 kDa protein isoforms of CD45. CD45 is encoded by the PTPRC (Protein tyrosine phosphatase receptor type C) gene. CD45, also known as the leukocyte common antigen (LCA), is a member of the protein tyrosine phosphatase (PTP) family. It is present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and thymocytes. CD45 is absent from circulating erythrocytes, platelets, or mature erythroid cells of bone marrow and non-hemopoietic tissues.
- This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
FORMAT
Format
FITC
Excitation Source
Blue 488 nm
Excitation Max
494 nm
Emission Max
520 nm
- FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
Other Formats →
APC
APC-R700
Alexa Fluor® 700
BB515
BUV395
BUV805
BV421
BV480
BV510
BV605
BV650
BV711
BV786
Biotin
PE
PE-CF594
PE-Cy™5
PE-Cy™7
PerCP-Cy™5.5
Purified
SUGGESTED COMPANION PRODUCTS
Cat No. | Description | Size | |
| | 100 Tests |
|
- 窗体顶端
- 窗体底端
RESOURCES & TOOLS
Spectrum Viewer
Panel Designer
Download TDS
PREPARATION AND STORAGE
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
PRODUCT NOTICES
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
公司简介
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