BD Pharmingen™ 561865 FITC Mouse Anti-Human CD45
产品编号 561865 | BD Pharmingen™
Clone HI30
产品描述
Brand
BD Pharmingen™
Alternative Name
PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5
Vol. Per Test
5 µl
Isotype
Mouse IgG1, κ
Reactivity
Human (QC Testing)
Application
Flow cytometry (Routinely Tested)
Immunogen
Human Peripheral Blood Leucocytes
Workshop No.
IV N816
Storage Buffer
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status
RUO
产品详情
Description
The HI30 monoclonal antibody specifically binds to the 180, 190, 205, 220 kDa protein isoforms of CD45. CD45 is encoded by the PTPRC ( Protein tyrosine phosphatase receptor type C) gene. CD45, also known as the leukocyte common antigen (LCA), is a member of the protein tyrosine phosphatase (PTP) family. It is present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and thymocytes. CD45 is absent from circulating erythrocytes, platelets, or mature erythroid cells of bone marrow and non-hemopoietic tissues.
Format
Format
FITC
Excitation Source
Blue 488 nm
Excitation Max
494 nm
Emission Max
520 nm
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
Other Formats →
ØAPC
ØAPC-R700
ØAlexa Fluor® 700
ØBB515
ØBUV395
ØBUV805
ØBV421
ØBV480
ØBV510
ØBV605
ØBV650
ØBV711
ØBV786
ØBiotin
ØPE
ØPE-CF594
ØPE-Cy™5
ØPE-Cy™7
ØPerCP-Cy™5.5
ØPurified
Suggested Companion Products
Resources & Tools
ØSpectrum Viewer
ØPanel Designer
ØDownload TDS
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Product Notices
1 This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
2 Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3 Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
4 For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5 Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6 Source of all serum proteins is from USDA inspected abattoirs located in the United States.
7 An isotype control should be used at the same concentration as the antibody of interest.
公司简介
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在一百多年的发展进程中，BD以其雄厚的实力相继收购了近20家医疗器械和医疗设备公司，扩大了公司经营规模，丰富了产品线，提升了综合竞争能力，为世界同行所瞩目。
BD公司的业务共分为三大类: BD医疗, BD生物科学, BD诊断
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