Takara Clontech PN016 PrimerArray® Embryonic Stem Cells (Mouse)
产品编号 PN016 | Takara Clontech
RT-PCR Primers: PrimerArray Series for Embryonic Stem Cell and iPS Cell Screening
Gene expression analysis to screen for pluripotency of embryonic stem cell and induced pluripotent stem cell (iPS cell) is streamlined and efficient with the PrimerArray Embryonic Stem Cell array. This array includes primers for key markers of mouse or human embryonic stem cell pluripotency as determined by the International Stem Cell Initiative International (ISCI). When performing gene expression analysis by real-time RT-PCR, these ready-to use primer sets save time, minimize pipetting, and reduce error.
The PrimerArray Embryonic Stem Cell array contains 96 primer pairs representing 88 genes associated with embryonic stem cell or iPS pluripotency and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. The expression levels of multiple genes can be screened simultaneously. The PrimerArray Analysis Tool Ver. 2.2, available for download on this web page, and/or Multiplate RQ (for use with the Thermal Cycler Dice Real Time Systems, not available in all geographic locations) are useful for analyzing data obtained using these products. Primer set information and the PrimerArray analysis tool can be downloaded from this page (click on the Resources tab, then expand the drop-down menu under Technical Notes).
产品描述
Features
ØEasily investigate the pluripotency of ES/iPS cells by real-time PCR: Measure key markers of human or mouse embryonic stem cells as determined by the International Stem Cell Initiative International (ISCI)
ØAffordable: Each PrimerArray Set supplies sufficient qPCR primers for up to 12 real-time PCR runs (25 µL reaction volume)
ØReduces error by minimizing pipetting
ØEnables high-throughput analysis of stem cell pluripotency
ØOptimized for superior performance with SYBR Ex TaqqPCR kits
ØStraightforward data analysis with the PrimerArray Analysis Tool
ØCompatible with all standard real-time PCR systems
Applications
ØPluripotency screens on embryonic stem cell and iPS cell samples
ØScreening for expression of stem cell marker genes in response to conditions or agents affecting pluripotency
Procedural Overview for Stem Cell Gene Expression Analysis by Real Time RT-PCR
The overall workflow for the PrimerArray Sets is straightforward:
1 RNA extraction(30 min): We recommend NucleoSpin RNA II kits (Clontech Cat.#740955.50) or RNAiso (Cat.# 9108, not available in all geographic locations.)
2 Reverse transcription(20 min): We recommend PrimeScript RT Master Mix (Perfect Real Time) (Cat.# RR036A)
3 Dispense real-time PCR reaction solution(10 min): Prepare a master mix of your cDNA sample and SYBR Premix Ex TaqII (Tli RNase Plus) (Cat.# RR820A) and dispense into a 96-well plate.
4 Add PrimerArray primers(5 min): Use 4 µL per reaction
5 Real-time PCR(1 - 2 hours): Compatible with all commonly used real-time PCR systems
6 Data analysis(30 minutes): PrimerArray Analysis Tool makes data analysis easy
PrimerArray Analysis Tool
The PrimerArray analysis tool facilitates data analysis and can be downloaded from this page (click on the Resources tab, then expand the drop-down menu under Technical Notes). For the PrimerArray Analysis Tool v2.2 User Manual, click on the Documents tab, then expand the drop-down menu under User Manuals.
Primer Information for RT-PCR Primer Sets
To download primer information for each PrimerArray RT-PCR primer set, click on the Resources tab, then expand the drop-down menu under Technical Notes.
公司简介
美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美**家，也是亚裔创办的**的分子生物公司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。1999年被BD收购，在分子生物学研究领域已经建立了坚实的地位。2005年，日本Takara生物公司收购了BD生物科学公司的Clontech部，成为Takara在美国建立的全资子公司。
美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
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