Clone: | GK1.5 (See other available formats) |
Isotype: | Rat IgG2b, κ |
Isotype Control: | |
Reactivity: | Mouse |
Immunogen: | Mouse CTL clone V4 |
Formulation: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
Preparation: | The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC. |
Concentration: | 0.5 mg/ml |
Storage & Handling: | The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
Application: | |
Recommended Usage: | |
Excitation | Blue Laser (488 nm) |
Laser: | |
Application | Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3,in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, and immunoprecipitation1,2. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody (Cat. No. 100506), but not RM4-4 antibody (Cat. No. 116002). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100416). For in vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). |
Notes: | |
Application | 1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP) |
References: | 2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete) |
| 3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim) |
| 4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block) |
| 5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete) |
| 6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med.149:1286. (Deplete) |
| 7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete) |
| 8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete) |
| 9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC) |
| 10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC) |
| 11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block) |
| |
RRID: | AB_312690 (BioLegend Cat. No. 100405) |
| AB_312691 (BioLegend Cat. No. 100406) |
Antigen Details | |
Description: | CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck. |
Other Names: | L3T4, T4 |
Structure: | Ig superfamily, 55 kD |
Distribution: | Majority of thymocytes, T cell subset |
Function: | TCR co-receptor, T cell activation |
Ligand Receptor: | MHC class II molecule |
Antigen | 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. |
References: | 2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579. |
| 3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645. |
GeneID: | 12504 |
UniProt: | |
Keywords: | FITC anti-mouse CD4, GK1.5, FITC, L3T4, T4, Mouse, Flow Cytometry, Immunology, Antibodies |
