Product Details | |
Clone: | 581(See other available formats) |
Isotype: | Mouse IgG1, |
Isotype Control: | PerCP/Cy5.5 Mouse IgG1, Isotype Ctrl |
Workshop | V MA27 |
Number: | |
Reactivity: | Human,Cross-Reactivity:Cynomolgus |
Formulation: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). |
Preparation: | The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody. |
Concentration: | Lot-specific (please contact technical support for concentration and total ug amount, or use our Lookup tool if you have a lot number.) |
Storage & Handling: | The antibody solution should be stored undiluted between 2℃ and 8℃, and protected from prolonged exposure to light Do not freeze. |
Application: | FC - Quality tested |
Recommended Usage: | Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 ul to 5 ul per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 ul staining volume or per 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more atwww.biolegend.com/testsize regarding the test size change.Cy and CyDye are registered trademarks of GE Healthcare. |
Excitation | Blue Laser (488 nm) |
Laser: | |
Application | The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6. |
Notes: | |
Application | 1. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press. |
References: | 2. Felschow DM, et al. 2001. Blood 97:3768. |
| 3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488. |
| 4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) |
| 5. Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC) |
| 6. Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF) |
聽 | Publication Library |
RRID: | AB_1937272 (BioLegend Cat. No. 343521) |
| AB_2228973 (BioLegend Cat. No. 343522) |
Antigen Details | |
Description: | CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands. |
Other Names: | Gp105-120, My10 |
Structure: | 105-120 kD single chain mucin-like glycoprotein |
Distribution: | Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts |
Function: | Cell adhesion |
Ligand Receptor: | L-selectin, E-selectin |
Antigen | 1. Krause DS, et al. 1996. Blood 87:1. |
References: | 2. Puri KD, et al. 1995. J. Cell Biol. 131:261. |
| 3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey. |
GeneID: | 947 |
UniProt: | View information about CD34 on UniProt.org |
Keywords: | PerCP/Cy5.5 anti-human CD34, 581, PerCP/Cy5.5, Gp105-120, My10, Human, Cross-Reactivity:Cynomolgus, Flow Cytometry, Immunology, Antibodies |