Product Details | |
Clone: | MC.028.83.76.242 (See other available formats) |
Isotype: | Mouse IgG1 |
Reactivity: | Human, Mouse, Rat, Dog, Primate |
Immunogen: | This monoclonal antibody was raised against a peptide immunogen comprising amino acids 2069-2087 of the LRRK2 protein in mice that did not express endogenous LRRK2 protein. |
Formulation: | Phosphate-buffered solution. |
Preparation: | The antibody was purified by affinity chromatography. |
Concentration: | 1 mg/ml |
Storage & Handling: | The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody. |
Application: | ELISA, IHC, WB |
Recommended Usage: | Each lot of this antibody is quality control tested by immunohistochemical staining. |
| The optimal working dilution should be determined for each specific assay condition. |
| • WB: 1:2000 |
| • ELISA: Recommended usage as capture antibody: 5-15 µg/mL |
| • IHC: 1:50-1:100 with either biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501) or polymer based detection systems such as ACUITYAdvanced Biotin Free Polymer Detection (Cat. No. 931001). |
| Tissue Sections: Formalin-fixed, paraffin-embedded tissues & frozen sections |
| Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as citrate buffer retrieval solution (Cat. No. 928501, 928601). |
| Incubation: 60 minutes at room temperature |
| Other applications not yet tested. |
Application | This antibody is effective in immunoblotting (WB), ELISA, and immunohistochemistry (IHC). |
Notes: | |
| Positive Control tissue: Human mid-brain or pancreas. |
| Estimated molecular weight: 250 kD |
| This antibody was generated with support from The Michael J. Fox Foundation. |
| The Michael J. Fox Foundation is dedicated to finding a cure for Parkinson's disease through an aggressively funded research agenda and to ensuring the development of improved therapies for those living with Parkinson's today. |
| To learn more about MJFF, please visit www.michaeljfox.org. |
RRID: | AB_2564739 (BioLegend Cat. No. 808201) |
Antigen Details | |
Description: | Leucine-rich repeat kinase 2 (LRRK2), is a member of the leucine-rich repeat kinase family. This protein also functions as a GTPase. Proteins with leucine-rich regions play a role in protein-protein interactions and mediate assembly of the cell's structural framework (cytoskeleton). Mutations in LRRK2 genes have been associated with late-onset Parkinson's disease. |
Other Names: | Leucine-rich repeat serine/threonine-protein kinase 2, augmented in rheumatoid arthritis 17, AURA17, DARDARIN, PARK8, RIPK7, ROCO2 |
GeneID: | 120892 |
UniProt: | View information about LRRK2 on UniProt.org |
Keywords: | Purified anti-LRRK2, MC.028.83.76.242, Purified, Leucine-rich repeat serine/threonine-protein kinase 2, augmented in rheumatoid arthritis 17, AURA17, DARDARIN, PARK8, RIPK7, ROCO2, Human, Mouse, Rat, Dog, Primate, ELISA, Immunohistochemistry, Western Blotting, Immunology, Antibodies |
