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Purified anti-Tubulin β 3 (TUBB3)
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Purified anti-Tubulin β 3 (TUBB3) 
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4662.00
4662.0
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品牌: Biolegend(Covance) 型号: 801202
规格: 250 ul

Biolegend  801202 Purified anti-Tubulin β 3 (TUBB3) Antibody

产品编号  801202| biolegend

产品描述


Product Details

Clone:
TUJ1 (See other available formats)
Isotype:
Mouse IgG2a
Reactivity:
Mammalian
Immunogen:
This antibody was raised against microtubules derived from rat brain.
Formulation:
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation:
The antibody was purified by affinity chromatography.
Concentration:
1 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application:
IHC, WB, IP, ICC
Recommended Usage:
Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.

WB: 1:1000*

IHC: 1:500 - 1:5000

ICC: 1:1000 - 1:5000

Tissue: Formalin-fixed Paraffin-embedded and Frozen sections

Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as citrate buffer retrieval solution (Cat. No. 928501, 928601)

Primary Incubation: 60 minutes at room temperature
Application
This antibody is effective in immunoblotting (WB), immunoprecipitation (IP), immunohistochemistry (IHC), and immunocytochemistry (ICC).
Notes:

*Predicted MW = 50 kD

This antibody is well characterized and highly reactive to neuron specific Class III β-tubulin (βIII). TUJ1 does not identify β-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues5.
Application
1. Jouhilahti E, Peltonen S, Peltonen J. Class III β-Tubulin Is a Component of the Mitotic Spindle in Multiple Cell Types.Journal of Histochemistry & Cytochemistry; 56, 1113 - 1119, 2008. [IHC, ICC, WB] PubMed  
References:
2. Mozzetti S, Ferlini C, Concolino P, Filippetti F, Raspaglio G, Prislei S, Gallo D, Martinelli E, Ranelletti FO, Ferrandina G, Scambia G. Class III beta-tubulin overexpression is a prominent mechanism of paclitaxel resistance in ovarian cancer patients. Clin. Cancer Res. 11(1):298-305, 2005.

3. Brown NL, Patel S, Brzezinski J, Glaser T. Math5 is required for retinal ganglion cell and optic nerve formation.Development 128(13):2497-2508, 2001.

4. Jepsen K, Hermanson O, Onami TM, Gleiberman AS, Lunyak V, McEvilly RJ, Kurokawa R, Kumar V, Liu F, Seto E, Hedrick SM, Mandel G, Glass CK, Rose DW, Rosenfeld MG. Combinatorial roles of the nuclear receptor corepressor in transcription and development. Cell 102(6):753-763, 2000.

5. Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195.(WB)  

6. Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF)PubMed  

7. Barry D, et al.; Expansion of Neurofilament Medium C Terminus Increases Axonal Diameter Independent of Increases in Conduction Velocity or Myelin Thickness. J. Neurosci.; 32: 6209 - 6219, 2012. [WB] PubMed  

8. Ferletta M, et. al. 2011. Int. J. Cancer 129:45. (WB)PubMed

9. Wirt SE, et al. G1 arrest and differentiation can occur independently of Rb family function. J. Cell Biol., 191: 809-825, Nov 2010. [IHC] PubMed  

10. Cesca F, et al. 2012. Cell Death Differ. 2:194. (IF, WB)PubMed  

11. Kim KK, et al. 2013. J. Cell Biol. 4:443. (IF, WB) PubMed

12. Kim K, et al. 2015. PLOS ONE. 10: 0142693. PubMed

Publication Library
RRID:
AB_2313773 (BioLegend Cat. No. 801201)

AB_10063408 (BioLegend Cat. No. 801202)
Antigen Details

Description:
Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.
Other Names:
CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin<mms-435p-100,mms-435p-250 style="font-family: Arial, Helvetica, sans-serif; font-size: 14px; color: rgb(51, 51, 51);">
GeneID:
10381
UniProt:
View information about Tubulin beta 3 (TUBB3) on UniProt.org
Keywords:
Purified anti-Tubulin β 3 (TUBB3), TUJ1, Purified, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin<mms-435p-100,mms-435p-250 style="font-family: Arial, Helvetica, sans-serif; font-size: 14px; color: rgb(51, 51, 51);">, Mammalian, Immunohistochemistry, Western Blotting, Immunoprecipitation, Immunocytochemistry, Immunology, Antibodies


公司简介

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