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Dynabeads® Human T-Activator CD3/CD28 for T Cell Expansion and Activation
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Dynabeads® Human T-Activator CD3/CD28 for T Cell Expansion and Activation 
价格:
24846.00
24846.0
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品牌: Gibco/Invitrogen
型号: 11132D
规格: 5 x 2 mL
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Gibco/Invitrogen 11132D  Dynabeads® Human T-Activator CD3/CD28 for T-Cell Expansion and Activation

产品编号  11132D | Gibco/Invitrogen

产品描述

  • Dynabeads® Human T-Activator CD3/CD28 are for the activation and expansion of human T cells. Advantages of Dynabeads® Human T-Activator CD3/CD28:• Activation of T cells without the need for feeder cells• Activated cells that retain in vivo-like function• Maximum reproducibility without contamination by soluble antibodies or mitogens• From 100- to 1,000-fold T cell expansionAbout Dynabeads® Human T-Activator CD3/CD28Dynabeads® Human T-Activator CD3/CD28 offer a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The uniform 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet. For expansion of antigen-specific T cells from T cell clones or T cell lines, we recommend using Dynabeads® Human T-Activator CD3/CD28/CD137.Starting samplesStart with mononuclear cells (MNCs)/peripheral blood mononuclear cells (PBMCs) from either whole blood or buffy coat, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells.Downstream applicationsThe activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.Learn more about Dynabeads® products• Find Dynabeads® products for a whole range of applications.• Find magnets for Dynabeads® separations.
  • 规格

  • Cell Type:T Cells (Whole Population)
  • Clonality:Monoclonal
  • Final Product:Cells
  • Product Size:5 x 2 mL
  • Reactivity:Human
  • Sample Type (Specific):Cell Cultures, PBMC
  • Target Species:Human
  • Shipping Condition:Room Temperature
  • 内容及储存

  • 5 × 2 ml Dynabeads® Human T-Activator CD3/CD28 is supplied as a suspension containing 4 × 107 Dynabeads®/ml in phosphate buffered saline (PBS), pH 7.4, w/0.1% human serum albumin (HSA).
  • Storage Conditions: 2°C to 8°C. Do not freeze.
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