Gibco/Invitrogen 11161D Dynabeads® Human T-Activator CD3/CD28 for T-Cell Expansion and Activation

产品编号  11161D | Gibco/Invitrogen

产品描述

• Dynabeads® Human T-Activator CD3/CD28 are for the activation and expansion of human T cells. Advantages of Dynabeads® Human T-Activator CD3/CD28:• Activation of T cells without the need for feeder cells• Activated cells that retain in vivo-like function• Maximum reproducibility without contamination by soluble antibodies or mitogens• From 100- to 1,000-fold T cell expansionAbout Dynabeads® Human T-Activator CD3/CD28Dynabeads® Human T-Activator CD3/CD28 offer a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The uniform 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet. For expansion of antigen-specific T cells from T cell clones or T cell lines, we recommend using Dynabeads® Human T-Activator CD3/CD28/CD137.Starting samplesStart with mononuclear cells (MNCs)/peripheral blood mononuclear cells (PBMCs) from either whole blood or buffy coat, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells.Downstream applicationsThe activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.Learn more about Dynabeads® products• Find Dynabeads® products for a whole range of applications.• Find magnets for Dynabeads® separations.

规格

• Cell Type:T Cells (Whole Population)
• Clonality:Monoclonal
• Final Product:Activated, expanded T cells
• Form:Bead
• High Throughput Compatibility:Not High Throughput-Compatible (Manual)
• Host Species:Mouse
• Isolation Method:Dynabeads Untouched or Dynabeads FlowComp
• Ligand:Antibody
• Number of Reactions:200 Tests
• Product Size:0.4 mL
• Reactivity:Human
• Surface Functionality:Epoxy
• Target Species:Human
• Shipping Condition:Room Temperature

内容及储存

The product contains 0.4 ml Dynabeads Human T-Activator CD3/CD28 in phosphate buffered saline (PBS), pH 7.4, containing 0.1% human serum albumin (HSA). Store at 2–8°C

4 × 107 beads/ml suspension.

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