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重组SARS-CoV-2 S蛋白RBD(Fc Tag)(Recombinant SARS-Cov-2 Spike Protein-RBD(Fc Tag)) 
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品牌: 同立海源 型号: TL-762
规格: 100ug

重组SARS-CoV-2 S蛋白RBD(Fc Tag),GMP级

英文名称:Recombinant SARS-Cov-2 Spike Protein-RBD(Fc Tag)

产品描述(Description)

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

分子别名(Synonym)

2019-nCoV RBD Protein; 2019-nCoV S1 Protein; 2019-nCoV Spike RBD Protein

纯度(Purity)

≥95%采用SDS-PAGE凝胶分析和SEC-HPLC分析

内毒素(Endotoxin)

≤0.01EU/ug(凝胶法)

表达宿主(Expression host)

 

CHO细胞

分子量(Molecular Weight)

重组SARS-CoV-2 (2019-nCoV) RBD蛋白包含470个氨基酸,预测的理论分子量为52.9kd

蛋白序列(Protein Sequence)

DNA序列编码重组SARS-CoV-2 (2019-nCoV)RBD蛋白表达带有Fc标签在C末端

提示(Tips)

本蛋白带有人Fc标签,应用中会与人源抗体的二抗发生反应,因此请特别注意

提纯方法(Purification Method)

层析纯化

稳定性(Stability)

1、冻干的样本可在4℃保存24个月,溶解后的液体可于-20℃保存6-12个月,并且避免反复冻融。

2、液体样本在-20~-80℃保存12个月,融化后需要进行分装保存于-20℃保存6-12个月,避免反复冻融。


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