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UltraGRO™-PURE GI (heparin-free, validated gamma irradiated) 
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品牌: Helios 型号: HPCHXCGLI50
规格: 500mL

UltraGRO™ Research grade

Product Type:Cell culture supplement

Attribute:Xeno-free

Grade:Research grade

Product Name:UltraGRO™

Catalog Number:HPCPLCRL50, HPCPLCRL10, HPCPLCRL05


  • Description:

    UltraGRO™ cell culture supplement is a xeno-free, animal serum-free, and heparin-requiring supplement for replacing FBS (fetal bovine serum) to support cell expansion from research use. UltraGRO™ contains abundant growth factors and cytokines necessary for research or industrial cell growth and proliferation of multiple cell types (e.g. MSCs) in research-scale production.

  • Quality Control Test:

    1. Appearance: Yellow to Buff liquid

    2. Total Protein: 4.0 - 8.0 g/dL

    3. Endotoxin: ≤ 10 EU/mL

    4. Sterility: Pass

    5. Mycoplasma: Negative

    6. pH: Tested

    7. Osmolality: Tested

    8. Performance Test: Test and record

  • Storage and Shipping Information:

    UltraGRO™ is most stable when stored frozen until needed. The recommended storage temperature is -20°C. Thaw frozen UltraGRO™ product in 37°C water bath before use. Once UltraGRO™ is thawed, it is recommended to fully use for completed medium preparation (e.g. 5%) the same day, or to divide it into single-use aliquots and store unused aliquots at -20°C.

  • Packaging Information:

    500mL, 100mL, 50mL plastic bottles

  • Media Form:

    Frozen Liquid

  • Application:

    For human ex-vivo tissue and cell culture processing applications.

  • Instructions for Use:

    1. UltraGRO™ shows optimal growth of MSC at 5% (v/v) in typical cell culture media, i.e. α-MEM, which contains 2mM L-Glutamine as final concentrate.

    2. We recommend seeding MSCs at approximately 3x103~6x103 per cm2.

    3. UltraGRO™ requires heparin at a final concentration of 2 IU/mL to be added in the culture media when supplemented with 5% UltraGRO™. Failure to add heparin will result in coagulation during cell culture in typical media.

  • Cell Lines:

    bone marrow mesenchymal stem cells, adipose tissue derived mesenchymal stem cells, umbilical cord derived mesenchymal stem cells, other mesenchymal stem cells.

  • Important Information:

    1. Clotting or insoluble particles may form in thawed UltraGRO™. Published research has shown that particles will not alter the performance of the product.

    2. Clotting or insoluble particles may form in thawed UltraGRO™, it is recommended to centrifuge at 3,400 xg for 3~5 minutes or to filter the liquid concentrate with a sterile 40 μm Cell Strainer to remove insoluble particles.

    3. Filtering the completed medium (e.g. 5%), after UltraGRO™ is diluted in the basal medium, will not affect UltraGRO™ supplemented cell culture performance. However, 0.22 μm filtering is NOT recommended for the 100% UltraGRO™ concentrate, as this may reduce 5% UltraGRO™ cell culture performance.

    4. Repeated freeze-thaw cycles should be avoided as they will cause an increase in insoluble precipitates and resulting potential decrease in UltraGRO™ performance.

  • Application:

    For human ex-vivo tissue and cell culture processing applications.

  • Instructions for Use:

    1. UltraGRO™ shows optimal growth of MSC at 5% (v/v) in typical cell culture media, i.e. α-MEM, which contains 2mM L-Glutamine as final concentrate.

    2. We recommend seeding MSCs at approximately 3x103~6x103 per cm2.

    3. UltraGRO™ requires heparin at a final concentration of 2 IU/mL to be added in the culture media when supplemented with 5% UltraGRO™. Failure to add heparin will result in coagulation during cell culture in typical media.

  • Cell Lines:

    bone marrow mesenchymal stem cells, adipose tissue derived mesenchymal stem cells, umbilical cord derived mesenchymal stem cells, other mesenchymal stem cells.

  • Important Information:

    1. Clotting or insoluble particles may form in thawed UltraGRO™. Published research has shown that particles will not alter the performance of the product.

    2. Clotting or insoluble particles may form in thawed UltraGRO™, it is recommended to centrifuge at 3,400 xg for 3~5 minutes or to filter the liquid concentrate with a sterile 40 μm Cell Strainer to remove insoluble particles.

    3. Filtering the completed medium (e.g. 5%), after UltraGRO™ is diluted in the basal medium, will not affect UltraGRO™ supplemented cell culture performance. However, 0.22 μm filtering is NOT recommended for the 100% UltraGRO™ concentrate, as this may reduce 5% UltraGRO™ cell culture performance.

    4. Repeated freeze-thaw cycles should be avoided as they will cause an increase in insoluble precipitates and resulting potential decrease in UltraGRO™ performance.

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重庆市寰宇安泰生物科技有限公司【Chongqing Uante Biological Technology Co.,Ltd.】专注于生命科学科研试剂、耗材、仪器仪表等销售与科研技术服务。主营产品覆盖分子生物学、细胞生物学、免疫学、生物制药、诊断试剂研发、干细胞研究、美容抗衰等众多领域。代理或经销数十家知名品牌产品,如:OriGenStemCell、同立海源、CSTI、GEBPALLSigmaThermo FisherLonzaBD等。将为广大科研用户在生命科学研究领域,提供高品质科研产品服务。公司秉承“诚信为本、服务至上”的管理理念,在未来的工作中,不断努力,奋发进取,力争成为您的科研物资战略合作优选伙伴!为生命科学领域的一线工作者提供*强有力的基础与支撑。

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