【Product Description】
Ham's Nutrient Mixtures were originally developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured.
Ham's Nutrient Mixture F12 was originally designed for serial propagation and cloning of two CHO cell lines namely, CHD-3 and CHL-1 and mouse L cells. It is the medium of choice for the growth of cells of rodent origin and for cloning of myeloma and hybridoma cells. This medium is also the medium of choice for clonal toxicity assay using CHO cells.
Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
【Composition】
IngredientsMg/L
INORGANIC SALTS
Calcium chloride dihydrate44.100
Copper sulphate pentahydrate0.0025
Ferric nitrate nonahydrate0.834
Magnesium sulphate anhydrous
57.650
Potassium chloride223.600
Sodium chloride7599.000
Sodium bicarbonate1176.000
Sodium dihydrogen phosphate anhydrous142.040
Zinc sulphate tetrahydrate0.863
AMINO ACIDS
Glycine7.500
L-Alanine8.910
L-Arginine hydrochloride
210.700
L-Asparagine anhydrous15.010
L-Aspartic acid13.300
L-Cystine dihydrochloride
35.120
L-Glutamic acid14.700
L-Glutamine146.000
L-Histidine hydrochloride monohydrate22.960
L-Isoleucine3.940
L-Leucine13.100
L-Lysine hydrochloride36.500
L-Methionine4.480
L-Phenylalanine4.960
L-Serine10.500
L-Threonine11.900
L-Tryptophan2.040
L-Tyrosine Disodium Salt7.810
L-Valine11.700
VITAMINS
Biotin0.0073
Choline chloride13.960
D-Ca Pantothenate0.480
Folic acid1.320
Nicotinamide0.037
Pyridoxal hydrochloride0.062
Riboflavin0.038
Thiamine hydrochloride0.340
Vitamin B121.360
i-Inositol18.000
OTHERS
D-Glucose
1801.600
Hypoxanthine Sodium Salt4.770
Linoleic acid0.084
Lipoic acid0.210
Phenol red Sodium Salt1.240
Putrescine dihydrochloride0.161
Sodium pyruvate110.100
Thymidine0.730
【Storage and Stability】
Nutrient Mixture F-12 Ham, With L-Glutamine should be kept 2 8°C. The product is light sensitive and therefore should not be left in the light. When stored in the dark under ideal conditions, the product is stable until the expiry date.
As with any other liquid media formulations, deterioration of liquid media may be recognized by any of the following characteristics, among others including:
(a). Color Change;
(b). Presence of clumping/ flocculent debris/ granulation/ particulates precipitates or sediments;
(c). Insolubility;
(d). And/or decrease in expected perf ormance parameters.
Any material described above should not be used and therefore discarded.
【Procedure】
1.Take a bottle from the defined storage conditions at 2 8°C and read the label.
2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
3.Using aseptic/sterile technique under a laminar flow culture hood, work according to established protocols.
4.Antibiotics may be added if desired.
【Quality Control】
Nutrient Mixture F-12 Ham, With L-Glutamine is tested for sterility , pH, O smolality and endotoxin concentrations. In addition, each batch is tested for cell growth using A549.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
