【Product Description】
DMEM , High Glucose, without Sodium Pyruvate, with L-Glutamine is one of the most widely used modification of Eagle's medium.
DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine that contains four fold concentration of amino acids and ferric nitrate. The original formulation contains 1000mgs glucose per litre and was originally used to culture embryonic mouse cells.
DMEM high glucose is a further modification of original DMEM and contains 4500mgs glucose per litre. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines.
It is super low endotoxin SLETM grade，Endotoxin≤0.01EU/ml by LAL method. The production process and quality management system meet the requirements of cGMP and has passed ISO13485 certification.
It does not contain trace elements. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
【Composition】
IngredientsMg/L
INORGANIC SALTS
Calcium chloride dihydrate265.000
Ferric nitrate nonahydrate0.100
Magnesium sulphate anhydrous
97.720
Potassium chloride400.000
Sodium chloride6400.000
Sodium dihydrogen phosphate anhydrous109.000
AMINO ACIDS
Glycine30.000
L-Arginine hydrochloride84.000
L-Cystine dihydrochloride
62.570
L-Glutamine584.000
L-Histidine hydrochloride monohydrate42.000
L-Isoleucine105.000
L-Leucine105.000
L-Lysine hydrochloride146.000
L-Methionine30.000
L-Phenylalanine66.000
L-Serine42.000
L-Threonine95.000
L-Tryptophan16.000
L-Tyrosine Disodium Salt103.790
L-Valine94.000
VITAMINS
Choline chloride4.000
D-Ca Pantothenate 4.000
Folic acid4.000
Nicotinamide4.000
Pyridoxal hydrochloride4.000
Riboflavin0.400
Thiamine hydrochloride4.000
i-Inositol7.200
OTHERS
D-Glucose
4500.000
Phenol red sodium salt15.900
Sodium bicarbonate3700.000
【Storage and Stability】
DMEM , High Glucose, without Sodium Pyruvate, with L-Glutamine should be kept 2-8°C. The product is light -sensitive and therefore should not be left in the light. When stored in the dark under ideal conditions, the product is stable until the expiry date.
As with any other liquid media formulations, deterioration of liquid media may be recognized by any of the following characteristics, among others including:
(a). Color Change;
(b). Presence of clumping/flocculent debris/ granulation/ particulates\ precipitates or sediments;
(c). Insolubility;
(d). And/or decrease in expected performance parameters.
Any material described above should not be used and therefore discarded.
【Procedure】
1.Take a bottle from the defined storage conditions at 2-8°C and read the label.
2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
3.Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
4.Antibiotics may be added if desired.
【Quality Control】
DMEM, High Glucose, without Sodium Pyruvate, with L-Glutamine is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using A549.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
