【Product Description】
Minimum Essential Medium Eagle (MEM) is a modification of Basal Medium Eagle (BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. MEM can be used either with Earle's salts or Hank's salts and can also be additionally supplemented with non-essential amino acids (NEAA). This medium can be further modified by eliminating calcium to facilitate growth of cells in suspension cultures.
It is super low endotoxin SLETM grade，Endotoxin≤0.01EU/ml by LAL method. The production process and quality management system meet the requirements of cGMP and has passed ISO13485 certification.
It does not contain deoxyribonucleosides and ribonucleosides. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
【Composition】
IngredientsMg/L
INORGANIC SALTS
Calcium chloride dihydrate265.000
Magnesium sulphate anhydrous
97.720
Potassium chloride400.000
Sodium chloride6800.000
Sodium dihydrogen phosphate anhydrous122.000
AMINO ACIDS
Glycine50.000
L-Alanine
25.000
L-Arginine hydrochloride
126.000
L-Asparagine monohydrate
50.000
L-Aspartic acid
30.000
L-Cysteine hydrochloride
100.000
L-Cystine dihydrochloride
31.300
L-Glutamic acid75.000
L-Glutamine292.000
L-Histidine hydrochloride monohydrate42.000
L-Isoleucine52.000
L-Leucine52.000
L-Lysine hydrochloride72.500
L-Methionine15.000
L-Phenylalanine32.000
L-Proline40.000
L-Serine25.000
L-Threonine48.000
L-Tryptophan10.000
L-Tyrosine Disodium Salt51.900
L-Valine46.000
VITAMINS
Choline chloride1.000
D-Biotin0.100
D-Ca Pantothenate 1.000
Folic acid1.000
L-Ascorbic acid
50.000
Nicotinamide1.000
Pyridoxal hydrochloride1.000
Riboflavin0.100
Thiamine hydrochloride1.000
Vitamin B121.360
i-Inositol
2.000
OTHERS
D-Glucose
1000.000
Lipoic acid
0.200
Sodium pyruvate110.000
Sodium bicarbonate2200.000
【Storage and Stability】
MEM-Alpha should be kept 2-8°C. The product is light -sensitive and therefore should not be left in the light. When stored in the dark under ideal conditions, the product is stable until the expiry date.
As with any other liquid media formulations, deterioration of liquid media may be recognized by any of the following characteristics, among others including:
(a). Color Change;
(b). Presence of clumping/flocculent debris/ granulation/ particulates\ precipitates or sediments ;
(c). Insolubility;
(d). And/or decrease in expected performance parameters.
Any material described above should not be used and therefore discarded.
【Procedure】
1.Take a bottle from the defined storage conditions at 2-8°C and read the label.
2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
3.Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
4.Antibiotics may be added if desired.
【Quality Control】
Minimum Essential Medium-Alpha, with L-Glutamine ，without phenol red is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using Vero.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
