【Product Description】
Iscove's Modified Dulbecco's Medium is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium contains additional amino acids, sodium selenite, sodium pyruvate, vitamins and inorganic salts. Potassium nitrate is substituted by ferric nitrate. IMDM was the first medium utilizing HEPES buffer. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions.
【Composition】
IngredientsMg/L
INORGANIC SALTS
Calcium chloride dihydrate219.000
Disodium hydrogen phosphate anhydrous
109.000
Magnesium sulphate anhydrous97.730
Potassium chloride330.000
Potassium nitrate0.076
Sodium chloride4505.000
Sodium selenite
0.0173
AMINO ACIDS
Glycine30.000
L-Alanine
25.000
L-Arginine hydrochloride
84.000
L Asparagine25.000
L-Aspartic acid
30.000
L-Cystine dihydrochloride
91.240
L-Glutamic acid75.000
L-Glutamine584.000
L-Histidine hydrochloride monohydrate42.000
L-Isoleucine104.800
L-Leucine104.800
L-Lysine hydrochloride146.200
L-Methionine30.000
L-Phenylalanine66.000
L-Proline40.000
L-Serine42.000
L-Threonine95.200
L-Tryptophan16.000
L-Tyrosine Disodium Salt104.200
L-Valine93.600
VITAMINS
Choline chloride4.000
D-Biotin0.013
D-Ca-Pantothenate4.000
Folic acid4.000
Nicotinamide4.000
Pyridoxal hydrochloride4.000
Riboflavin0.400
Thiamine hydrochloride4.000
Vitamin B120.013
i-Inositol7.200
OTHERS
D-Glucose4500.000
Phenol red sodium salt15.000
Sodium pyruvate110.000
sodium bicarbonate3024.000
HEPES5958.000
【Storage and Stability】
Iscove's Modified Dulbecco's Medium (IMDM), with L-Glutamine should be stored at 2-8°C. Use before the expiry date.
【Procedure】
1.Take a bottle from the defined storage conditions at 2-8°C and read the label.
2.Wipe the outside of the bottle with a
disinfectant solution such as 70% ethanol.
3.Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
4.Antibiotics may be added if desired.
【Quality Control】
Iscove's Modified Dulbecco's Medium (IMDM), with L-Glutamine is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using SP-2.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
