【Product Description】
Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the protein composition of cultured mammalian cells. MEM can be used either with Earle's salts or Hanks salts and can also be additionally supplemented with non-essential amino acids (NEAA). This medium can be further modified by eliminating calcium to facilitate growth of cells in suspension cultures.
【Composition】
IngredientsMg/L
INORGANIC SALTS
Calcium chloride dihydrate265.000
Magnesium sulphate anhydrous97.720
Potassium chloride400.000
Sodium chloride6800.000
Sodium phosphate monobasic anhydrous
122.000
AMINO ACIDS
L-Arginine hydrochloride
126.000
L-Cystine dihydrochloride
31.300
L-Glutamine292.000
L-Histidine hydrochloride monohydrate42.000
L-Isoleucine52.000
L-Leucine52.000
L-Lysine hydrochloride72.500
L-Methionine15.000
L-Phenylalanine32.000
L-Threonine48.000
L-Tryptophan10.000
L-Tyrosine Disodium Salt51.900
L-Valine46.000
VITAMINS
Choline chloride1.000
D-Ca-Pantothenate1.000
Folic acid1.000
Nicotinamide1.000
Pyridoxal hydrochloride1.000
Riboflavin0.100
Thiamine hydrochloride1.000
i-Inositol2.000
OTHERS
D-Glucose1000.000
Phenol red sodium salt11.000
Sodium pyruvate2200.000
【Storage and Stability】
Minimum Essential Medium Eagle (MEM), With Earle's salts and L-Glutamine should be stored at 2-8°C. Use before the expiry date.
【Procedure】
1.Take a bottle from the defined storage conditions at 2-8°C and read the label.
2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
3.Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
4.Antibiotics may be added if desired.
【Quality Control】
Minimum Essential Medium Eagle (MEM), With Earle's salts and L-Glutamine is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using Vero.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
