【Product Description】
McCoy's 5A medium was developed at Roswell Park Memorial Institute in Buffalo, New York. The first medium was developed in 1955 as the result of studies on the nutritional requirements of the Walker 256 carcinoma. The original formulation was based on the amino acids in concentrations similar to those in Eagle's medium as well as the water soluble vitamins of Medium199. Modifications to the original formulation resulted in the final version being published in 1960. The final formulation also incorporates modifications done by Iwakata and Grace and contains increased amounts of folic acid, vitamin B12, and peptone. This medium is also known to support growth of primary cultures derived from a variety of tissues.
Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
【Composition】
IngredientsMg/L
INORGANIC SALTS
Calcium chloride dihydrate132.430
Magnesium sulphate anhydrous97.720
Potassium chloride400.000
Sodium chloride6460.000
Sodium phosphate monobasic anhydrous
504.350
AMINO ACIDS
Glycine7.510
L-Alanine13.360
L-Arginine hydrochloride42.140
L-Asparagine, anhydrous45.030
L-Aspartic acid19.970
L-Cysteine hydrochloride31.500
L-Glutamic acid22.070
L-Glutamine219.200
L-Histidine hydrochloride20.960
L-Hydroxyproline19.670
L-Isoleucine39.360
L-Leucine39.360
L-Lysine hydrochloride36.540
L-Methionine14.920
L-Phenylalanine16.520
L-Proline17.270
L-Serine26.280
L-Threonine17.870
L-Tryptophan3.060
L-Tyrosine Disodium Salt26.100
L-Valine17.570
VITAMINS
Ascorbic acid 0.56250.5625
Biotin0.200
Choline chloride5.000
D-Ca-Pantothenate0.200
Folic acid10.000
Niacin0.500
Niacinamide0.500
Pyridoxal hydrochloride0.500
Pyridoxine hydrochloride0.500
Riboflavin0.200
Thiamine hydrochloride0.200
Vitamin B122.000
i-Inositol36.000
p-Amino benzoic acid (PABA)1.000
OTHERS
D-Glucose3000.000
Glutathione （reduced）0.500
Peptic digest of animal tissue600.000
Phenol red (Sodium Salt)11.000
Sodium bicarbonate2200.000
【Storage and Stability】
McCoy's 5A Medium（(Modified)）, with L-Glutamine should be stored at 2-8°C. Use before the expiry date.
【Procedure】
1.Take a bottle from the defined storage conditions at 2-8°C and read the label.
2.Wipe the outside of the bottle with a
disinfectant solution such as 70% ethanol.
3.Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
4.Antibiotics may be added if desired.
【Quality Control】
McCoy's 5A Medium（(Modified)）, with L-Glutamine is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using Vero.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
