【Product Description】
Medium 199 was the first nutritionally defined medium developed by Morgan, Morton, and Parker in 1950.This complex medium was formulated specifically for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. It was observed that explanted tissue could survive in Medium 199 without serum but long term cultivation of cells required supplementation of the medium with serum.
Medium 199 is formulated with either Hank's salts or Earle's salts. The medium when supplemented with serum can be used for growth of a wide variety of cells. Medium 199 is presently used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells.
Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
【Composition】
IngredientsMg/L
INORGANIC SALTS
Calcium chloride dihydrate265.000
Magnesium sulphate anhydrous97.720
Potassium chloride400.000
Sodium acetate anhydrous50.000
Sodium chloride6800.000
Sodium phosphate monobasic anhydrous
122.000
AMINO ACIDS
Ascorbic acid0.050
Glycine50.000
L-Alanine
25.000
L-Arginine hydrochloride
70.000
L-Aspartic acid
30.000
L-Cysteine hydrochloride monohydrate0.100
L-Cystine dihydrochloride
26.000
L-Glutamic acid67.000
L-Glutamine100.000
L-Histidine hydrochloride monohydrate22.000
L-Hydroxyproline10.000
L-Isoleucine20.000
L-Leucine60.000
L-Lysine hydrochloride70.000
L-Methionine15.000
L-Phenylalanine25.000
L-Proline40.000
L-Serine25.000
L-Threonine30.000
L-Tryptophan10.000
L-Tyrosine Disodium Salt57.660
L-Valine25.000
VITAMINS
Calciferol0.100
Choline chloride0.500
D-Biotin0.010
D-Ca-Pantothenate0.010
DL-Tocopherol phosphate disodium salt0.010
Folic acid0.010
Menadione0.010
Nicotinamide0.025
Nicotinic acid0.025
Pyridoxal hydrochloride0.025
Pyridoxine hydrochloride0.025
Retinol Acetate0.140
Riboflavin0.010
Thiamine hydrochloride0.010
i-Inositol0.050
p-Amino benzoic acid (PABA)0.050
OTHERS
Adenine sulphate10.000
Adenosine monophosphate0.200
Adenosine triphosphate1.000
Cholesterol0.200
Deoxyribose0.500
Ferric nitrate nonahydrate0.720
Glucose1000.00
Glutathione reduced0.050
Guanine hydrochloride0.300
Hypoxanthine0.354
Phenol red sodium salt15.000
Polysorbate 804.900
Ribose0.500
Thymine0.300
Uracil0.300
Xanthine0.344
sodium bicarbonate2200.000
【Storage and Stability】
Medium 199, With Earle's salts and L-Glutamine should be kept 2-8°C. The product is light -sensitive and therefore should not be left in the light. When stored in the dark under ideal conditions, the product is stable until the expiry date.
As with any other liquid media formulations, deterioration of liquid media may be recognized by any of the following characteristics, among others including:
(a). Color Change;
(b). Presence of clumping/flocculent debris/ granulation/ particulates\ precipitates or sediments ;
(c). Insolubility;
(d). And/or decrease in expected performance parameters.
Any material described above should not be used and therefore discarded.
【Procedure】
1.Take a bottle from the defined storage conditions at 2-8°C and read the label.
2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
3.Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
4.Antibiotics may be added if desired.
【Quality Contro】
Medium 199, With Earle's salts and L-Glutamine is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using Vero.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
