STEMdiff™ Neural Progenitor Medium
Defined, Medium for maintenance and expansion of neural progenitor cells derived from human ES and iPS cells
- 产品描述
STEMdiff™ Neural Progenitor Medium is a defined and serum-free medium for the expansion of neural progenitor cells (NPCs) derived from human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium (Catalog #05835). NPCs cultured in this medium can be expanded 3-5 fold per passage, and cultured for at least 10 passages, with minimal spontaneous neuronal differentiation.
Advantages:
• Defined and serum-free • Supports expansion of NPCs generated using STEMdiff™ Neural Induction Medium • Optimized for efficient expansion of NPCs over multiple passages • Preserves NPC multipotency while minimizing spontaneous neuronal differentiation • Convenient, user-friendly format and protocol
Components:
STEMdiff™ Neural Progenitor Medium (Catalog #05833)
STEMdiff™ Neural Progenitor Basal Medium, 500 mL
STEMdiff™ Neural Progenitor Supplement A (50X), 10 mL
STEMdiff™ Neural Progenitor Supplement B (1000X), 500 µL
Subtype:专业培养基
Cell Type:PSC衍生神经细胞; 神经干细胞和祖细胞; 多能干细胞
Species:人
Application:细胞培养; 扩增
Brand:STEMdiff
Area of Interest:疾病模型; 药物发现和毒性测试; 神经科学; 干细胞生物学
Formulation:成分确定; 无血清
- Product Applications
- This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
hPSC-Derived Neural Cell Research
Neural Induction
Expansion
Differentiation and Maturation
Characterization
Data and Publications
Data
- Figure 1. Morphology and Marker Expression of Neural Progenitor Cells Cultured in STEMdiff™ Neural Progenitor Medium
- (A) Typical NPC morphology is observed in cultures (shown at day 6 of passage 1). (B-D) NPCs maintained in STEMdiff™ Neural Progenitor Medium express the CNS-type NPC markers PAX6 (B, D, red), SOX1 (C, red) and NESTIN (C, green), but not the neural crest marker SOX10 (D, green, single channel shown in inset). B-D were taken at the same magnification.
- Figure 2. Expansion of Neural Progenitor Cells in STEMdiff™ Neural Progenitor Medium
- NPCs cultured in STEMdiff™ Neural Progenitor Medium can be expanded to generate a large number of cells. Three- to five-fold expansion can be achieved upon each passage. NPCs were derived using STEMdiff™ Neural Induction Medium and passaged once a week on average. n = 6.
- Figure 3. Neural Progenitor Cells Cultured in STEMdiff™ Neural Progenitor Medium Show Minimal Spontaneous Neuronal Differentiation
- Passages 1 (A) and 3 (B) of a representative NPC culture maintained in STEMdiff™ Neural Progenitor Medium. Cells were immunolabeled with SOX1 (red) to identify NPCs, and class III β-tubulin (green) to identify neurons. Spontaneous neuronal differentiation is low in NPC cultures maintained in STEMdiff™ Neural Progenitor Medium. A and B were taken at the same magnification.
- Figure 4. Neural Progenitor Cells Maintained in STEMdiff™ Neural Progenitor Medium can Differentiate into Neurons and Astrocytes
- When directed according to published protocols, NPCs can differentiate into neurons (A, class III β-tubulin shown in red) and astrocytes (B, GFAP shown in red). Nuclei are counterstained with DAPI (blue).
- Related Products
- STEMdiff™ Neural Rosette Selection Reagent
Enzyme-free reagent for the selective detachment of neural rosette structures
- BrainPhys™ Neuronal Medium
Serum-free neurophysiological basal medium for improved neuronal function
- STEMdiff™ Neural Induction Medium
Defined, serum-free medium for neural induction of human ES and iPS cells
- STEMdiff™ Neural Progenitor Freezing Medium
For cryopreservation of neural progenitor cells generated using STEMdiff™ Neural Induction Medium
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