- MesenCult™-SF Culture Kit
- Defined, serum-free culture kit for human mesenchymal stem cells
- 产品描述
MesenCult™-SF Culture Kit is a standardized, serum-free system for the culture of human mesenchymal stem cells (MSCs). It consists of MesenCult™-XF Medium (Catalog #05420) and MesenCult™-SF Attachment Substrate (Catalog #05424) and is optimized for the expansion of MSCs in vitro as well as their enumeration using the colony-forming unit - fibroblast (CFU-F) assay. This product also supports long-term growth of MSCs and cells maintain multi-lineage differentiation potential. The MesenCult™-SF Culture Kit must be used in conjunction with the MesenCult™-ACF Dissociation Kit (Catalog #05426). Components of the MesenCult™-ACF Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are passaged and cultured with MesenCult™-XF Medium. MesenCult™-XF Medium must be supplemented with L-glutamine.
Components:
MesenCult™-SF Culture Kit (Catalog #05429)
MesenCult™-XF Basal Medium, 400 mL (Catalog #05421)
MesenCult™-XF Supplement (5X), 100 mL (Catalog #05422)
MesenCult™-SF Attachment Substrate, 5 mg (Catalog #05424)
Subtype:专业培养基
Cell Type:间充质干细胞和祖细胞
Species:人
Application:细胞培养; 集落检测; 扩增
Brand:MesenCult
Area of Interest:干细胞生物学
Formulation:成分确定; 无血清
- Product Applications
- This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Mesenchymal Stem and Progenitor Cell Research
Cell Sourcing and Isolation
Culture and Cryopreservation
Differentiation
Characterization
Data and Publications
Data
- Figure 1. Human bone marrow-derived MSCs cultured in MesenCult™-XF expand faster than cells cultured in a traditional serum-based medium (n = 3; Mean ± SEM)
- Figure 2. Human bone marrow-derived MSCs cultured in MesenCult™-XF display multi-lineage differentiation potential
- A. Oil red O staining of adipocytes generated from passage 1 MSCs previously cultured in MesenCult™-XF.B. Alizarin Red detection of Ca2+ deposits indicates the formation of bone structures in cells generated from passage 4 MSCs previously cultured in MesenCult™-XF.C. Alcian Blue staining of chondrocytes generated from passage 2 MSCs previously cultured in MesenCult™-XF.D. Collagen II staining of chondrocytes generated from passage 2 MSCs previously cultured in MesenCult™-XF.
- Figure 3. Primary human bone marrow-derived MSCs show less hematopoietic cell contamination when cultured in (A) MesenCult™-XF compared to a (B) traditional serum-based medium
- Passage 0 human bone marrow-derived MSCs cultured in MesenCult™-XF (A) or a traditional serum-based medium (B).
- Figure 4. Phenotype of culture-expanded human MSCs cultured in MesenCult™-XF.
- Figure 5. MSCs cultured in MesenCult™-XF suppress T cell proliferation and reduce cell cycle division more robustly than MSCs cultured in a traditional serum-based medium
- Passage 2 MSCs generated in MesenCult™-XF or a traditional serum-based medium were treated with mitomycin C prior to co-culture with T cells. T cells were purified from peripheral blood using EasySep™ (Catalog #19051) immunomagnetic separation and fluorescently labeled using 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (5(6)CFDA,SE; CFSE). 2 x 105 CFSE-labeled T cells were cultured with 1 x 105 MSCs in serum-free medium supplemented with 100 U/mL IL-2. T cells were stimulated with tetrameric antibody complexes against CD3ε, CD28 and CD2. On days 3 and 7, cells were harvested, stained with anti-CD45 antibody and propidium iodide and the T cell division history measured as CFSE dye dilution analyzed by flow cytometry.
- Related Products
- MesenCult™-XF Medium
Defined, xeno-free medium for human mesenchymal stem cells
- MesenCult™-ACF Freezing Medium
Animal component-free MSC cryopreservation medium
- MesenCult™-SF Attachment Substrate
Serum-free attachment substrate for human mesenchymal stem cells
- MesenCult™-ACF Culture Kit
Defined, animal component-free culture kit for human mesenchymal stem cells
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