Stemcell 18765RF EasySep™ Mouse CD4+CD62L+ T Cell Isolation Kit
Product Overview
Isolate highly purified naïve CD4+ T cells from single-cell suspensions of splenocytes or other tissues by using a simple, two-step method. First, naive CD4+ T cells are pre-enriched by negative selection using the EasySep™ Mouse Naïve CD4+ T Cell Pre-Enrichment Cocktail containing biotinylated antibodies directed against non-naïve CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD25, CD45R, CD49b, TCRγ/δ, TER119). Then CD4+CD62L+ cells, pre-labeled with CD62L PE, are selected using the EasySep™ PE Selection Cocktail. The desired cells are labeled with antibodies and magnetic particles. The cells are separated without columns using an EasySep™ magnet.
For the isolation of mouse naïve CD4+ T cells in as little as 15 minutes by negative selection, we recommend using the EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (19765).
Advantages:• Fast and easy-to-use
• Up to 97% purity
• No columns required
Components:
EasySep™ Mouse CD4+CD62L+ T Cell Isolation Kit (Catalog #18765)
EasySep™ Mouse Naïve CD4+ T Cell Pre-Enrichment Cocktail, 0.5 mL
EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL
EasySep™ PE Positive Selection Cocktail, 1 mL
EasySep™ Dextran RapidSpheres™ 50100, 1 mL
Normal Rat Serum, 2 mL
RoboSep™ Mouse CD4+CD62L+ T Cell Isolation Kit (Catalog #18765RF)
EasySep™ Mouse Naïve CD4+ T Cell Pre-Enrichment Cocktail, 0.5 mL
EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL
EasySep™ PE Positive Selection Cocktail, 1 mL
EasySep™ Dextran RapidSpheres™ 50100, 1 mL
Normal Rat Serum, 2 mL
RoboSep™ Buffer (Catalog #20104)
RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:细胞分选试剂盒
Cell Type:T细胞; CD4+T细胞
Species:小鼠
Sample Source:其他; 脾
Selection Method:正选
Application:细胞分离
Brand:EasySep; RoboSep
Area of Interest:免疫学
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