Stemcell 19058 EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion
Product Overview
The EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion is designed to isolate monocytes and CD14+CD16+ monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. The CD14+CD16+ subset of monocytes (~10% in blood of healthy individuals) has characteristics of tissue macrophages and expands greatly in acute and chronic inflammatory disease. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-monocyte cells and magnetic particles. The cocktail also contains and antibody to human Fc receptor to minimize nonspecific binding. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which removal of all CD16+ cells is desired, we recommend the EasySep™ Human Monocyte Enrichment Kit (Catalog #19359), which contains anti-CD16.
Advantages:• Fast, easy-to-use and column-free
• Up to 81% purity
• Untouched, viable cells
Components:
EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion (Catalog #19058)
EasySep™ Human Monocyte Enrichment Cocktail without CD16 Depletion, 1 mL
EasySep™ Magnetic Particles, 1 mL
RoboSep™ Human Monocyte Enrichment Kit without CD16 Depletion with Filter Tips (Catalog #19058RF)
EasySep™ Human Monocyte Enrichment Cocktail without CD16 Depletion, 1 mL
EasySep™ Magnetic Particles, 1 mL
RoboSep™ Buffer (Catalog #20104)
RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyPlate™ EasySep™ Magnet (Catalog 18102)
• RoboSep™-S (Catalog #21000)
Subtype:细胞分选试剂盒
Cell Type:单核细胞
Species:人
Sample Source:PBMC
Selection Method:负选
Application:细胞分离
Brand:EasySep; RoboSep
Area of Interest:免疫学
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