Overview
The EasySep™ HLA CD2 Positive Selection Kit is designed to isolate CD2+ cells from fresh or previously frozen human peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD2 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD2 antigen is expressed on T cells and NK cells. Positively selected cells are compatible with flow cytometric crossmatch analysis (when used with EasySep™ HLA FCXM Blocking Solution) and any other downstream assay.
Advantages:
• Fast and easy-to-use
• Up to 98% purity
• No columns required
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Area of Interest:
嵌合体; HLA; 免疫学
