Overview
This kit is meant for selection and cloning applications where the hygromycin B antibiotic is used as the selective agent. ClonaCell™-TCS Medium is a serum-containing, methylcellulose-based semi-solid medium that can be used for selection and cloning of a variety of suspension-adapted and adherent cell lines, such as BHK-1, CHO-K1, CHO-S, CHO-DG44, and HEK-293. This medium contains pre-selected fetal bovine serum (FBS) and bovine serum albumin (BSA). Hygromycin B is suitable for use in cell culture for the selection and maintenance of hygromycin-resistant clones when using ClonaCell™-TCS Medium.
Advantages:
• Supports high cloning efficiency and robust colony formation for a variety of cell lines such as: CHO-K1, CHO-S, HEK-293, B16F-10, BaF/3, BHK-1, FD-5, Jurkat Daudi, Molt-4, UT-7, K562
Components:
• ClonaCell™-TCS Medium, 80 mL (Catalog #03814)
• Hygromycin, 100 mg (Catalog #03813)
Contains:
• IMDM
• Methylcellulose
• Pre-selected serum
• 2-Mercaptoethanol
• Bovine serum albumin
• L-glutamine
• Other ingredients
Cell Type:
CHO细胞; HEK-293; 其他
Application:
抗体开发; 细胞培养; 细胞系制备
Area of Interest:
细胞系制备; 半固体克隆
