Overview
The ClonaCell™-HY kit contains media and reagents for all steps in mouse monoclonal antibody production. The ClonaCell™-HY method uses a methylcellulose-based semi-solid selective medium to combine the hybridoma selection and cloning into one step. Single cell-derived hybridomas stay together in the semi-solid medium as they grow to form monoclonal colonies. The hybridomas can easily be picked and screened, and then expanded for production of the desired antibody. This kit is suitable for use during development of monoclonal antibodies from a variety of host animals, including human, mouse, rat, hamster, and rabbit.
Advantages:
• Time savings: Hybridoma selection and cloning are combined into one step
• Resource savings: Single-cell derived hybridomas form visible discrete colonies in semi-solid medium, and are easy to pick and screen, and transfer to liquid medium for expansion
• Cloning efficiency: Individual cells are suspended and immobilized in semi-solid medium, preventing loss of rare, high-producing clones by overgrowth
Contains:
• DMEM
• Pre-selected serum
• Gentamycin
• 2-Mercaptoethanol
• Supplements
• L-glutamine
• Other ingredients
• Some kit components contain Hypoxanthine, Aminopterin, Thymidine (HAT)
Application:
抗体开发; 细胞培养; 细胞系制备; 杂交瘤生成
Area of Interest:
细胞系制备; 药物发现和毒性测试; 杂交瘤生成; 半固体克隆
