Overview
ClonaCell™-HY Medium D is a semi-solid methylcellulose-based medium containing the selective reagents hypoxanthine, aminopterin, and thymidine (HAT). This medium is used after fusion of splenocytes and myeloma cells to select and clone hybridomas in one step. Single cell-derived hybridomas stay together in the semi-solid medium as they grow to form monoclonal colonies. The hybridomas can easily be picked and screened, and then expanded for production of the desired antibody. This medium is suitable for use during development of monoclonal antibodies from a variety of host animals, including human, mouse, rat, hamster, and rabbit.
Benefits of semi-solid cloning:
• Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated.
• Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution.
• Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures.
Advantages:
• Time savings: Hybridoma selection and cloning are combined into one step
• Resource savings: Single-cell derived hybridomas form visible discrete colonies in semi-solid medium, and are easy to pick and screen, and transfer to liquid medium for expansion
• Cloning efficiency: Individual cells are suspended and immobilized in semi-solid medium, preventing loss of rare, high-producing clones by overgrowth
Contains:
• DMEM
• Methylcellulose
• Pre-selected serum
• Hypoxanthine, aminopterin, and thymidine (HAT)
• Gentamycin
• 2-Mercaptoethanol
• L-Glutamine
• Supplements
• Other ingredients
Application:
抗体开发; 细胞培养; 细胞系制备; 杂交瘤生成
Area of Interest:
癌症研究; 细胞系制备; 药物发现和毒性测试; 杂交瘤生成; 半固体克隆
