Overview
ClonaCell™-TCS Medium is a serum-containing methylcellulose-based semi-solid medium that can be used for selecting and cloning a variety of suspension-adapted cell lines, including CHO-S and hybridomas. It can also be used for semi-solid cloning of some cell lines that grow adherently in the presence of serum, including CHO-K1, BHK-1, and HEK-293. This medium contains pre-selected fetal bovine serum (FBS) and bovine serum albumin (BSA) and supports robust growth of a wide variety of cell types. ClonaCell™-TCS Medium does not contain selection agents.
Benefits of semi-solid cloning:
• Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated.
• Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution.
• Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures.
Advantages:
• Supports high cloning efficiency and robust colony formation for a variety of cell lines such as: CHO-K1, CHO-S, HEK-293, B16F-10, BaF/3, BHK-1, FD-5, Jurkat Daudi, Molt-4, UT-7, K562
Contains:
• IMDM
• Pre-selected serum
• Bovine serum albumin
• L-Glutamine
• 2-Mercaptoethanol
• Methylcellulose
• Other ingredients
Cell Type:
CHO细胞; HEK-293; 杂交瘤; 其他
Application:
抗体开发; 细胞培养; 细胞系制备
Area of Interest:
细胞系制备; 杂交瘤生成; 半固体克隆
